Randomized trial assesses transient GFP expression using geminivirus-derived replicons in tobacco and tomato, indicating enhanced protein production capabilities.
Key Points
This study aims to evaluate the effectiveness of geminivirus-derived replicons on transient GFP expression in two plant species.
Evaluated three geminiviral backbones: BeYDV, ToLCV, WDV against a non-replicating T-DNA control
GFP constructs delivered via Agrobacterium tumefaciens and circularization verified in planta
GFP RNA and protein levels assessed by RT–qPCR, fluorescence imaging, and ELISA.
In tobacco, BeYDV increased GFP transcript levels by 221-fold at 6 dpi, with approximately fivefold higher protein levels.
ToLCV and WDV enhanced transcript levels up to 6.3-fold in tomato and protein levels by 2.4-fold.
GVRs significantly improved transient gene expression relative to control, with performance varying by replicon and plant species.
Cite This Study
Τσακιρπάλογλου et al. (2026) studied this question.