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June 6, 2026International Journal of Molecular SciencesOpen Access

Inhibitory Effects and Mode of Action of Pure Eugenol Versus Clove Essential Oil on Key Phytopathogenic Fungi

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Authors

FSF. SempereJRJosefa RosellóMSMaría Pilar Santamarina

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Overview

Randomized trial evaluates antifungal efficacy in vitro, suggesting eugenol is more effective than clove essential oil.

Key Points

  • This research aims to determine whether eugenol or clove essential oil exhibits superior antifungal activity against key phytopathogenic fungi.
  • Characterization of clove essential oil using GC-FID and GC-MS.
  • In vitro evaluation of antifungal activity against Botryotinia fuckeliana, Rhizoctonia solani, and Verticillium dahliae at 300 µg/mL.
  • Data analyzed using two-way ANOVA followed by Tukey’s HSD test.
  • Eugenol exhibited higher antifungal activity than clove essential oil across all tested fungi.
  • Complete inhibition (100%) of V. dahliae was achieved with eugenol; clove essential oil showed lower efficacy.
  • Despite 87.3% eugenol content in the essential oil, its activity was less effective, indicating potential modulation by minor oil constituents.

Cite This Study

Sempere et al. (2026) studied this question.

synapsesocial.com/papers/6a23ba3c71a5da9775e75f37https://doi.org/10.3390/ijms27115083
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