March 15, 1999Journal of Clinical InvestigationOpen Access

A vascular bed–specific pathway regulates cardiac expression of endothelial nitric oxide synthase

Q: What is the clinical evidence from this study?

Study design: Other. Population: eNOS gene regulation. Intervention: 1600-bp eNOS promoter coupled to LacZ / Conditioned media from cardiac myocytes vs. Control media / other cell types. Primary outcome: eNOS promoter induction (luciferase activity / LacZ expression).

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Key result

The eNOS gene is regulated in the cardiac endothelium by both a PDGF-dependent and PDGF-independent microvascular bed-specific signaling pathway.

Population

Transgenic mice with the 1,600-bp 5' flanking region of the human eNos promoter coupled to the coding region…

Design

Preclinical

Authors

PGPascale V. GuillotUniversity College London JGJason GuanBrigham and Women's Hospital LLLixin LiuShanghai University

Discussion

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Implication

Supports microvascular bed-specific eNOS regulation in cardiac endothelium; leaves open translation to human disease or therapy.

Structured PICO

Population

Transgenic mice and murine embryonic endothelial progenitor cells were used to study the transcriptional regulation of the eNOS gene by vascular bed-specific signaling pathways.

Intervention

Conditioned media from cardiac myocytes, skeletal myocytes, and brain astrocytes; neutralizing anti-platelet-derived growth factor (PDGF) antibodies; PDGF-AB

Outcome

eNos promoter activity and transgene expression (beta-galactosidase activity)surrogate

The eNos gene is regulated in the cardiac endothelium by both PDGF-dependent and PDGF-independent microvascular bed-specific signaling pathways.

Limitations

In vitro models may not fully replicate in vivo conditions
Transgene expression may be influenced by the site of genomic integration

Cite This Study

Guillot et al. (1999) studied eNOS gene regulation. 1600-bp eNOS promoter coupled to LacZ / Conditioned media from cardiac myocytes vs. Control media / other cell types was evaluated on eNOS promoter induction (luciferase activity / LacZ expression). The eNOS gene is regulated in the cardiac endothelium by both a PDGF-dependent and PDGF-independent microvascular bed-specific signaling pathway.

synapsesocial.com/papers/6a23ceb7a98279854dd36bdf https://doi.org/10.1172/jci6017

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A vascular bed–specific pathway regulates cardiac expression of endothelial nitric oxide synthase

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

A vascular bed–specific pathway regulates cardiac expression of endothelial nitric oxide synthase

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

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