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August 29, 2023Cardiovascular ResearchOpen Access

AngII-induced hypertensive heart disease in mice impairs β-AR heart rate responses via PDE4D upregulation.

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Why the study?

Chronotropic incompetence is common in hypertensive heart disease, but the underlying basis for this is poorly understood.

Does PDE4D knockdown or inhibition improve heart rate responses to β-adrenergic stimulation in mice with AngII-induced hypertensive heart disease?

Population

C57BL/6 mice infused with saline or AngII

Comparison

AngII infusion vs saline infusion

Design

Preclinical animal study

Follow-up

3 weeks

Key result

AngII-induced hypertensive heart disease in mice impaired heart rate responses to β-AR stimulation due to up-regulation of PDE4D and reduced cAMP effects in sinoatrial node myocytes.

Authors

TDTristan W. DoreyMMMegan D. McRaeDBDarrell D. Belke

Discussion

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Member takes

Overview

Hypothesis-generating for PDE4D inhibition in chronotropic incompetence; extends mechanistic insights in hypertensive models but requires human validation.

Structured PICO

Does PDE4D knockdown or inhibition improve heart rate responses to β-adrenergic stimulation in mice with AngII-induced hypertensive heart disease?

P
Population
C57BL/6 mice with AngII-induced hypertensive heart disease studied for heart rate and sinoatrial node function.
I
Intervention
Knockdown of PDE4D using a virus-delivered shRNA or inhibition of PDE4 with rolipram
C
Comparator
Saline infusion (control) or untreated AngII-infused mice
O
Outcome
Heart rate (HR) and sinoatrial node (SAN) function in response to the β-AR agonist isoproterenol (ISO)surrogate

PDE4D up-regulation mediates impaired β-adrenergic receptor signaling and chronotropic incompetence in a mouse model of hypertensive heart disease, which can be reversed by PDE4D knockdown or inhibition.

Cite This Study

Dorey et al. (2023) studied AngII-induced hypertensive heart disease. Angiotensin II (AngII) vs. Saline was evaluated on Heart rate and sinoatrial node function in response to the β-AR agonist isoproterenol. AngII-induced hypertensive heart disease in mice impaired heart rate responses to β-AR stimulation due to up-regulation of PDE4D and reduced cAMP effects in sinoatrial node myocytes.

synapsesocial.com/papers/6a24187d4e11633d95ab3522https://doi.org/10.1093/cvr/cvad138
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Mechanisms of Sinoatrial Node Dysfunction in Heart Failure With Preserved Ejection Fraction2021 · 67 citations
  2. 2Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice2021 · 7 citations
  3. 3Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction2021 · 2 citations
  4. 4Cardiac Overexpression of PDE4B Blunts β-Adrenergic Response and Maladaptive Remodeling in Heart Failure2020 · 79 citations
  5. 5Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction2022 · 18 citations