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The regulatory mechanisms governing the biosynthesis of dietary fibers in the leaves and stems of mustard (Brassica juncea L.) have not been adequately characterized. Through quantitative trait locus (QTL) mapping, 23 consensus QTLs associated with dietary fiber content in rapidly growing leaves and stems were identified, highlighting Bju.CESA6.A01 as a Key Candidate Gene. Functional validation through overexpression of Bju.CESA6.A01 in Arabidopsis thaliana led to a significant 22.07% increase in the primary dietary fiber component, neutral detergent fiber. This enhancement was accompanied by increases in the number of vascular bundles, vascular bundle area, and proportion of vascular bundle area in stems. Additionally, 12 novel genes associated with Bju.CESA6.A01 was identified. These findings provide valuable insights into the genetic regulation of dietary fiber biosynthesis in mustard leaves and stems during their rapid growth phase.
Xu et al. (Thu,) studied this question.