What question did this study set out to answer?

The aim is to demonstrate the distribution of DPN diaphorase and other linked dehydrogenases using a new reagent.

A Histochemical Method for the Demonstration of Diphosphopyridine Nucleotide Diaphorase

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Histochemical investigation demonstrates DPN diaphorase distribution in rat organs, indicating enzyme specificity.

The aim is to demonstrate the distribution of DPN diaphorase and other linked dehydrogenases using a new reagent.
Developed Nitro-BT reagent for histochemical localization.
Used sections of rat kidney and stomach buffered at pH 7.4.
Conducted incubations at 37 degrees C for 15 minutes.
Beta-hydroxybutyric dehydrogenase was uniquely present in gastric mucosa and proximal convoluted tubule of the kidney.
Lactic dehydrogenase and DPN diaphorase were found in nearly all cell types in both organs.

Nachlas et al. (1958) studied this question.

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