What question did this study set out to answer?

To analyze the topography of ribosomal proteins by measuring alkylation patterns of lysine residues.

December 1, 1974Biochemical JournalOpen Access

Reductive alkylation of ribosomes as a probe to the topography of ribosomal proteins

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To analyze the topography of ribosomal proteins by measuring alkylation patterns of lysine residues.
Escherichia coli ribosomes treated with aldehydes and NaBH4.
Incorporation of (3)H or (14)C.
Two-dimensional polyacrylamide-gel electrophoresis for protein separation.
All ribosomal proteins in 30S and 50S subunits modified by alkylation.
Similar patterns of alkylation across different aldehydes.
Every ribosomal protein has at least one lysine residue exposed at the surface.

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Randomized trial investigates ribosomal protein exposure in Escherichia coli, suggesting insights into ribosome structure.

Moore et al. (1974) studied this question.

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