Development And HPLC-UV Validation of Tecoma Stans Ethanolic Leaf Gel For Antidiabetic and Wound Healing Activity

AbstractThis study concentrated on the development and assessment of a herbal topical gel usingethanolic extract of Tecoma stans for wound-healing purposes. The leaves of Tecoma stansunderwent Soxhlet extraction with ethanol, resulting in a phytochemical-dense extractcomprising flavonoids, phenolics, alkaloids, glycosides, and tannins. Quantitative analysisverified elevated levels of phenolics and flavonoids, signifying considerable antioxidantcapacity. Herbal gel formulations were developed utilizing HPMC K15M as the gelling agent.Among all formulations, batch F4, which contained 4% extract and 2.5% HPMC, demonstratedoptimal physicochemical qualities, including appropriate viscosity, homogeneity, spreadability,and skin-compatible pH. In vitro diffusion tests exhibited prolonged drug release over 8 hoursin accordance with Higuchi diffusion kinetics. Analytical standardization via UV–Visiblespectroscopy and HPLC validated the presence of quercetin, chlorogenic acid, and lapachol.Accelerated stability experiments demonstrated exceptional physical and chemical stability,with negligible alterations in drug content and rheological characteristics. The results indicatethat the formulated Tecoma stans herbal gel demonstrates significant potential as a stable andefficacious wound-healing agent.