Synthetic CpG oligonucleotides induce a genetic profile ameliorating murine myocardial I/R injury

Figures

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  Figure 1. Time course of experimental interventions and assessments. Nonlinear scale for better identification of various interventions. ODN = CpG/control oligodeoxynucleotide, LAD = left anterior descending artery

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  Figure 2. Prolonged up‐regulation of defined pathways 3 h towards 24 h post‐I/R is a CpG‐mediated effect. Figure shows heat map for up‐regulated pathways in control and CpG ODN‐treated animals, 3 and 24 h post‐I/R. Hierarchical clustering according to IPA definition. Heat map colour‐coded according to the z‐score (see 2 section)

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  Figure 3. Genes predicted to increase cardiovascular angiogenesis are strongly up‐regulated by CpG pre‐conditioning 24 h post‐I/R. (A) Network analysis of CpG ODN‐induced activation of angiogenic genes. Ingenuity Pathway Analysis was used to identify the network of regulatory genes (open labels) contributing to CpG ODN‐induced activation. (B) IL1 complex is one regulator of angiogenesis 24 h post‐I/R. Figure shows network of genes significantly induced by IL1 complex. These genes are known to increase cardiovascular angiogenesis (see also Figure 3A). Asterisks indicate that fold change values of replicates have been averaged

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  Figure 4. CpG‐mediated IL‐10 activity effectively down‐regulates target genes (3 h vs 24 h). Figure shows target genes of IL‐10 network in CpG‐treated animals. While 3 h post‐I/R, all genes were strongly up‐regulated, most genes experience down‐regulation towards 24 h post‐I/R. Asterisks indicate that fold change values of replicates have been averaged