What question did this study set out to answer?

The aim is to evaluate how tagging individual turnip mosaic virus proteins affects their function and viral pathogenicity.

June 10, 2026PathogensOpen Access

Contrasting Effects of Tagging Turnip Mosaic Virus Proteins

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Randomized trial measures viral infection impacts from tagging turnip mosaic virus proteins in plants, highlighting tag implications.

The aim is to evaluate how tagging individual turnip mosaic virus proteins affects their function and viral pathogenicity.
Tagged helper component proteinase (HC-Pro) and nuclear inclusion protein b (NIb) in a TuMV infectious clone.
Used constructs with a 6His-3xFLAG tag placed at various protein termini.
Assessed pathogenicity in Nicotiana benthamiana and Arabidopsis thaliana.
HC-Pro-tagged constructs maintained pathogenicity similar to TuMV-GFP.
NIb-tagged clones exhibited temperature-sensitive infectivity with reduced pathogenicity.
Structural analysis showed the tag interfered with NIb catalytic site but not with HC-Pro.

Gomaa et al. (2026) studied this question.

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