Randomized trial reveals the phylogenetic position of Amorphophallus allenii, suggesting evolutionary insights within the genus.
The chloroplast genome of Amorphophallus allenii, a rare species endemic to limestone habitats, was sequenced to resolve phylogenetic uncertainties within the genus. Using PacBio HiFi sequencing, we performed a de novo assembly and obtained a complete circular genome of 170,657 bp, featuring a typical quadripartite structure. The genome encodes 126 genes, including 81 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Maximum likelihood phylogenetic analyses based on whole plastome, coding sequence, and protein alignments robustly support the placement of A. allenii within the Continental Asia I clade, with A. muelleri identified as its closest relative. This study provides a reliable genomic resource that clarifies the species’ phylogenetic position and establishes a foundation for investigating evolutionary diversification and biogeographic patterns in the genus Amorphophallus.
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Shi et al. (2026) studied this question.
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