Randomized trial establishes a framework for respiratory microbiota transplantation, reshaping metabolic and immune characteristics in mice, indicating potential for human applications.
Key Points
This study aims to create an optimized framework for respiratory microbiota transplantation and assess its effects on metabolic and immune characteristics.
Developed a method for respiratory microbiota transplantation in murine models.
Evaluated sample storage, delivery routes, and treatment regimens using microbiome profiling.
Assessed the impact on metabolism and immunity through metabolomic and transcriptomic analysis.
Successfully established mouse-to-mouse respiratory microbiota transplantation using bronchoalveolar lavage fluid.
Transplantation partially restored 188 metabolites and 2721 host genes altered in germ-free mice.
Optimal administration of human BALF and sputum-derived microbiota was achieved via intratracheal and intranasal delivery methods.