Experimental evolution reveals how larval habitats shape insecticide resistance and biochemical responses in mosquitoes, suggesting implications for vector management.
Key Points
This research examines how different larval habitats influence insecticide resistance and metabolic enzyme plasticity in Anopheles gambiae.
Experimental evolution over 10 generations using An. gambiae larvae from Accra, Ghana.
Rearing in three water types: field-collected, dechlorinated tap, and distilled water.
Assessment of susceptibility to insecticides and molecular determination of resistance mutations.
kdr-w mutation increased to fixation by generation F2 in dechlorinated tap water, but declined to ~63% by F10.
ace-1 mutation increased from 60% to 90% over the generations.
Detoxification enzyme activity was higher in field-collected water, with oxidase levels elevated by 32% compared to other water types.