The Role of Gynostemma pentaphyllum Extract in Combating H ₂ O ₂ ‐Induced Oxidative Stress and Preventing Hair Graying

Objectives Hair graying, often linked to aging (oxidative stress), has gained significant attention due to its social implications and the desire for a youthful appearance. Currently, there is no effective solution. The objective of this study was to determine whether Gynostemma pentaphyllum (Thunb.) Makino extract (GP) can mitigate the effects of H 2 O 2 ‐induced oxidative stress in melanocytes and evaluate its potential as a treatment for hair graying. Methods Oxidative stress models were established using B16 melanoma cells and C57BL/6 mice. GP treatment’s effects on cell viability, melanocyte apoptosis, intracellular reactive oxygen species (ROS) levels, melanin production, tyrosinase activity, total superoxide dismutase (SOD) activity, and the Wnt/β‐catenin signaling pathway were assessed. In animal experiments, the protective effects of GP on mouse hair follicles were evaluated. Results GP treatment enhanced cell viability, decreased melanocyte apoptosis, reduced intracellular ROS levels, increased melanin production, boosted tyrosinase and SOD activity, and potentially modulated the Wnt/β‐catenin signaling pathway. In animal experiments, GP significantly protected mouse hair follicles from H 2 O 2 ‐induced damage. Conclusion The findings suggest that GP has potential as a treatment for hair graying by mitigating H 2 O 2 ‐induced oxidative stress in melanocytes, highlighting its therapeutic potential in addressing oxidative stress‐related hair graying.