Novel Robust-Thermostable Zearalenone Hydrolase Engineered via Ancestral Sequence Reconstruction: In Silico Design, In Vitro Characterization, and In Vivo Efficacy Evaluation

Yue Wang; Qianqian Wang; Xinlan Liu; Yingyi Xu; Yu Tang; Yongpeng Guo; Xin Fang; Gaigai Wang; Qiugang Ma; Lihong Zhao

doi:10.1021/acs.jafc.6c04812

June 10, 2026Journal of Agricultural and Food Chemistry

Novel Robust-Thermostable Zearalenone Hydrolase Engineered via Ancestral Sequence Reconstruction: In Silico Design, In Vitro Characterization, and In Vivo Efficacy Evaluation

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Key Points

This research aims to evaluate the efficacy of engineered enzyme An48 in degrading zearalenone.
In silico design of An48 through ancestral sequence reconstruction.
In vitro characterization to assess enzyme activity.
In vivo testing in mouse gastrointestinal tract.
An48 effectively degrades zearalenone in the gastrointestinal tract of mice, demonstrating notable efficacy.

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Implication

Evaluates zearalenone degradation by engineered enzyme in mice, indicating industrial applications.

Abstract

tests confirmed that An48 effectively degrades ZEN in the gastrointestinal tract of mouse. These results indicate that ancestral enzyme An48 shows promising industrial applications.

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Cite This Study

Wang et al. (2026) studied this question.

synapsesocial.com/papers/6a2ba2f68101cf8926f01c59 https://doi.org/10.1021/acs.jafc.6c04812

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Novel Robust-Thermostable Zearalenone Hydrolase Engineered via Ancestral Sequence Reconstruction: In Silico Design, In Vitro Characterization, and In Vivo Efficacy Evaluation

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Novel Robust-Thermostable Zearalenone Hydrolase Engineered via Ancestral Sequence Reconstruction: In Silico Design, In Vitro Characterization, and In Vivo Efficacy Evaluation

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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