This method measures β-amylase activity in plant seeds, suggesting improved specificity over traditional techniques.
In view of the disadvantages of the 3,5-dinitrosalicylic acid (DNS) method, we developed a rapid micro-volume procedure utilizing p-nitrophenyl-α-maltopentaoside (PNPG5) as a substrate for specific determination of β-amylase activity. The assay procedure was evaluated with seeds from 4 plant species, and the exact degree of interference by α-amylase was determined. This modified method could be applied to the selective measurement of β-amylase activity in different plant seeds.
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凌腾芳 et al. (2006) studied this question.
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