What does this research mean for the field?

The anti-mouse CCR1 monoclonal antibodies C1Mab-2 and C1Mab-6 bind to the N-terminal region of the receptor (amino acids 1-13), with residues Glu2, Asp5, and Phe6 being essential for C1Mab-2 recognition, and Glu2, Ile3, and Asp5 being crucial for C1Mab-6. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Identify the binding epitope of monoclonal antibodies C 1 Mab-2 and C 1 Mab-6 against mouse CCR1.

June 12, 2026Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method

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Authors

AOAyaka OkadaTohoku University HSHiroyuki SuzukiTohoku University MKMika K. KanekoTohoku University

Discussion

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Implication

Examines the binding epitope of monoclonal antibodies in mice, revealing essential recognition sites.

Key Points

Identify the binding epitope of monoclonal antibodies C 1 Mab-2 and C 1 Mab-6 against mouse CCR1.
Utilized flow cytometry for epitope mapping of monoclonal antibodies.
Analyzed mutants of mouse CCR1 with altered extracellular domains.
Conducted alanine scanning in the N-terminal region to identify critical amino acids.
C 1 Mab-2 and C 1 Mab-6 bind to the N-terminal region of mouse CCR1, particularly at amino acids 2-13.
Essential residues for C 1 Mab-2 recognition include Glu2, Asp5, and Phe6.
Essential residues for C 1 Mab-6 recognition include Glu2, Ile3, and Asp5.

Cite This Study

Okada et al. (2026) studied this question.

synapsesocial.com/papers/6a2ba31a8101cf8926f01d9d https://doi.org/10.1177/21679436261459606

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June 12, 2026Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Epitope Mapping of Anti-Mouse C-C Motif Chemokine Receptor 1 Monoclonal Antibodies Developed by the Cell-Based Immunization and Screening Method

View Full Paper

Ask AI

Bookmark

Authors

AOAyaka OkadaTohoku University HSHiroyuki SuzukiTohoku University MKMika K. KanekoTohoku University

Discussion

Member takes

Implication

Examines the binding epitope of monoclonal antibodies in mice, revealing essential recognition sites.

Key Points

Identify the binding epitope of monoclonal antibodies C 1 Mab-2 and C 1 Mab-6 against mouse CCR1.
Utilized flow cytometry for epitope mapping of monoclonal antibodies.
Analyzed mutants of mouse CCR1 with altered extracellular domains.
Conducted alanine scanning in the N-terminal region to identify critical amino acids.
C 1 Mab-2 and C 1 Mab-6 bind to the N-terminal region of mouse CCR1, particularly at amino acids 2-13.
Essential residues for C 1 Mab-2 recognition include Glu2, Asp5, and Phe6.
Essential residues for C 1 Mab-6 recognition include Glu2, Ile3, and Asp5.

Cite This Study

Okada et al. (2026) studied this question.

synapsesocial.com/papers/6a2ba31a8101cf8926f01d9d https://doi.org/10.1177/21679436261459606

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