Study of the Scavenging Radical Activities of Polysaccharide of Hippophae rhamnosides Fruit Peel

To study the scaventing radical activities of polysaccharide of Hippophae rhamnodides fruit peel, crude polysaccharide was isolated from the fruit peel of H. rhamnosides with hot water at 80 ℃, then precipitated by ethanol, and the protein in the extracts was removed by the Sevag method. The fractions of H₁、H₂ and H₃ of H. rhamnosides polysaccharide were obtained by adding 50% and 70% ethanol, respectively. The Fenton reaction was used to produce and detect hydroxyl radicals (·OH), and pyrogallol/EDTA/Tris-HCl was used to produce and detect superoxide anion (). H. rhamnosides polysaccharides had a strong scavenging capacity for both ·OH and oxygen radicals. When the fractional concentrations of H₁、H₂ and H₃ were at 200 µg·mL⁻¹, their scavenging rates to hydroxyl radicals were 44.9%, 49.0%, and 26.4%, respectively, and to superoxide anion 36.9%, 15.4%, and 23.1%, respectively. The scavenging rates to oxygen radicals increased with increasing the polysaccharide concentration; there appeared a proportional relation between effect and quantity.