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Demonstrates HBsAg gene expression in transgenic Arachis hypogaea, suggesting potential for vaccine development.
A plant expression vector carrying hepatitis B surface antigen (HBsAg) gene was constructed and used to transform Arachis hypogaea mediated by Agrobacterium tumefacient. Transgenic Arachis hypogaea plants were identified by PCR and southern-blot. ELISA assay showed that the HBsAg gene was expressed in transgenic Arachis hypogaea and had a expressing level of 2.41µg·g⁻¹ fresh leaves.
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Zhu et al. (2006) studied this question.
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