What question did this study set out to answer?

The aim is to establish a quadruplex real-time PCR assay for detecting four swine pathogens simultaneously.

June 12, 2026Transboundary and Emerging DiseasesOpen Access

The Quadruplex Real‐Time PCR Assay for Simultaneous Detection of GETV, PRRSV, JEV, and PCV2 in Swine

Q: What is the clinical evidence from this study?

Population: Swine viral infections (GETV, PRRSV, JEV, PCV2) (n=151). Intervention: Quadruplex real-time PCR assay vs. Established standard methods. Primary outcome: Concordance with established standard methods.

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Key result

New quadruplex PCR assay achieves 100% concordance with standard methods for GETV, PRRSV, JEV, and PCV2.

n=151

Why the study?

This study aimed to establish a quadruplex real-time PCR assay for the simultaneous detection of GETV, PRRSV, JEV, and PCV2.

Does a quadruplex real-time PCR assay accurately detect GETV, PRRSV, JEV, and PCV2 in swine samples compared to standard methods?

Key Points

The aim is to establish a quadruplex real-time PCR assay for detecting four swine pathogens simultaneously.
Developed specific primers and probes for GETV, PRRSV, JEV, and PCV2.
Conducted systematic optimization of the reaction system and established standard curves.
Evaluated specificity, sensitivity, and clinical application performance using 151 clinical samples.
Achieved positive detection rates of 18.54% for GETV, 14.57% for PRRSV, 3.31% for JEV, and 10.60% for PCV2.
No cross-reactivity observed with 12 common swine pathogens, confirming assay specificity.
Intra-assay and inter-assay coefficients of variation were below 1.4%, indicating high reliability.

Structured PICO

Does a quadruplex real-time PCR assay accurately detect GETV, PRRSV, JEV, and PCV2 in swine samples compared to standard methods?

Population

151 clinical swine samples tested to evaluate a quadruplex real-time PCR assay for simultaneous detection of GETV, PRRSV, JEV, and PCV2.

Exposure

Quadruplex real-time PCR assay for GETV, PRRSV, JEV, and PCV2

Comparator

Established standard methods

Outcome

Diagnostic performance (specificity, sensitivity, repeatability, and clinical application)surrogate

The developed quadruplex real-time PCR assay provides a highly sensitive and specific method for the rapid differential diagnosis of four common swine viruses.

Discussion

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Implication

Indicates notable GETV circulation in swine; leaves open multiplex PCR adoption pending larger validation studies.

Cite This Study

Wang et al. (2026) studied Swine viral infections (GETV, PRRSV, JEV, PCV2) (n=151). Quadruplex real-time PCR assay vs. Established standard methods was evaluated on Concordance with established standard methods. A newly developed quadruplex real-time PCR assay for detecting GETV, PRRSV, JEV, and PCV2 demonstrated 100% concordance with established standard methods in 151 clinical swine samples.

synapsesocial.com/papers/6a2bd11d6550ea4541ffe8e1 https://doi.org/10.1155/tbed/7291266

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The Quadruplex Real‐Time PCR Assay for Simultaneous Detection of GETV, PRRSV, JEV, and PCV2 in Swine

Key Points

Structured PICO

Discussion

Implication

Cite This Study

The Quadruplex Real‐Time PCR Assay for Simultaneous Detection of GETV, PRRSV, JEV, and PCV2 in Swine

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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