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June 13, 2026Frontiers in MicrobiologyOpen Access

Synergistic effects of biochar and Bacillus subtilis on soil fertility and tobacco leaf quality in acidified soil: microbial mechanisms and multi-model evaluation

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Authors

WHWeihua HuYunnan Agricultural UniversityZWZheng WangChina Tobacco Guangxi Industrial (China)QYQigang YangChina Tobacco Guangxi Industrial (China)

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Overview

Randomized trial evaluates soil fertility and tobacco quality impacts of biochar and Bacillus subtilis in acidified soils, suggesting effective management strategies.

Key Points

  • This research aims to investigate the synergistic effects of biochar and Bacillus subtilis on soil fertility and tobacco leaf quality in acidified soils.
  • Field study with five treatments: control, biochar alone, and biochar combined with Bacillus subtilis at varying rates.
  • Assessment of soil physicochemical properties, microbial community structure, and tobacco leaf quality.
  • High-throughput 16S rRNA sequencing and principal component analysis for comprehensive evaluation.
  • Biochar–microbe co-application significantly improved soil fertility metrics and tobacco leaf quality.
  • Both BF2 and BF3 yielded the highest improvements in leaf quality, with no significant difference between treatments.
  • Enzyme activities were enhanced, proving instrumental in mediating the effects on soil fertility and microbial function.

Cite This Study

Hu et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf2a9faef96ed7f0557aahttps://doi.org/10.3389/fmicb.2026.1842613
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