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June 13, 2026

The RecBCD complex interacts directly with the DNA sliding clamp in Escherichia coli.

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Authors

IRIda Mathilde Marstein RiisnæsSRSynnøve Brandt RæderSSSigne Simonsen

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Overview

Randomized trial reveals interaction between RecBCD complex and β-clamp, suggesting role in DNA repair mechanisms.

Key Points

  • This research aims to define the interaction between the β-clamp and the RecBCD complex in Escherichia coli.
  • Conducted bacterial two-hybrid assays and co-immunoprecipitation to examine interactions.
  • Used fluorescence microscopy and nuclear magnetic resonance spectroscopy to map the binding sites.
  • Analyzed the functionality of mutant RecB to assess the impact on DNA damage survival.
  • RecB associates with β-clamp, with the binding mapped to the ligand pocket.
  • Mutation of RecB residues 1018-1023 abolishes β-clamp binding and reduces cell survival after DNA damage.
  • Indications of a second binding site in the helicase domain of RecB were found.

Cite This Study

Riisnæs et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf41ffaef96ed7f0568f6https://doi.org/10.1093/nar/gkag570
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