Key result
Gel chromatography and LC-MS/MS enable preparative isolation of plasma LDL and HDL fractions.
This paper provides a detailed protocol for isolating LDL and HDL fractions from human blood plasma using gel chromatography, facilitating further research on plasma lipoproteins.
The pathogenesis of atherosclerosis, the main cause of cardiovascular disease, including coronary heart disease and stroke, involves plasma lipoproteins, which differ in protein and lipid composition, size, charge and function. At present, chromatographic methods, in particular gel filtration (SEC), are widely used to isolate lipoproteins. In this article, we present a protocol for the preparative isolation of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) fractions from human blood plasma using gel chromatography. To determine the boundaries of the elution zones of apolipoproteins A-1 and B-100, HDL and LDL markers, respectively, we used liquid chromatography with tandem mass spectrometry (LC-MS/ MS) with ELISA cross-validation. Lipoprotein fractions obtained with the help of the SEC can be used for various studies of scientific and applied importance.
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Kaluzhskiy et al. (2026) studied this question. Preparative isolation of LDL and HDL fractions using gel chromatography was evaluated. A protocol for the preparative isolation of low-density and high-density lipoprotein fractions from human blood plasma using gel chromatography and LC-MS/MS was developed.
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