New Protein Fndc5 - 252995 - 32872484-32862268 (Grch38) - Start_6212 - Codon_atg - 27aa - Plddt_78,0

This technical dossier presents the structural characterization and bioengineering properties of a novel open reading frame (DarkORF) associated with the FNDC5 genetic locus (Identifier: 252995). The nucleotide sequence is mapped at chromosomal coordinates 32872484-32862268 (GRCH38), precisely positioned inside Reading Frame +2 with an initialization point at 6212 bp. Computational translation yielded a continuous polypeptide chain of 27 amino acids, exhibiting a theoretical molecular weight of 3237,84 Da and an isoelectric point (pI) of 10,20. Advanced structural prediction modeling via AlphaFold/ColabFold architecture achieved a mean confidence score (pLDDT) of 78,0%. This exceptionally high score indicates a rigid, well-defined alpha-helix topological architecture with maximum atomic-level accuracy, suitable for immediate crystallographic validation. Thermodynamic benchtop screening classified the protein framework status as Estavel, validating its viability for industrial protein expression and synthetic gene optimization protocols in Escherichia coli systems.