New Protein FGF21 - 26291 - 48755524-48758330 (GRCH38) - START_1787 - CODON_ATG - 27AA - PLDDT 71

This technical dossier presents the structural characterization and bioengineering properties of a novel open reading frame (DarkORF) engineered via computational design as a synthetic analogue of the FGF21 genetic locus (Reference ID: 26291). The synthetic sequence was generated utilizing structural parameters modeled from chromosomal coordinates 48755524-48758330 (GRCH38), calibrated inside Reading Frame +2 relative to position 1787 bp. Computational translation yielded a continuous polypeptide chain of 27 amino acids, exhibiting a theoretical molecular weight of 2806,22 Da and an isoelectric point (pI) of 8,60. Advanced structural prediction modeling via AlphaFold/ColabFold architecture achieved a mean confidence score (pLDDT) of 71,0%. This exceptionally high score indicates a rigid, well-defined alpha-helix topological architecture with maximum atomic-level accuracy, suitable for immediate crystallographic validation. Thermodynamic benchtop screening classified the protein framework status as Estavel, validating its viability for industrial protein expression and synthetic gene optimization protocols in Escherichia coli systems.