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This study aims to validate a high-throughput RNA sequencing test for detecting plant viruses and viroids.

June 13, 2026VirusesOpen Access

Validation of an In-House High-Throughput Total RNA Sequencing Test for the Detection of Plant Viruses and Viroids

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Randomized trial demonstrates effective detection of plant viruses and viroids in symptomatic samples, indicating reliable diagnostics.

This study aims to validate a high-throughput RNA sequencing test for detecting plant viruses and viroids.
Developed an in-house workflow including total RNA extraction, ribodepletion, Illumina sequencing, and bioinformatics analyses.
Evaluated performance criteria such as analytical sensitivity, specificity, selectivity, repeatability, reproducibility, and robustness.
Focused on detection of specific viruses and viroids: tomato brown rugose fruit virus, tomato leaf curl New Delhi virus, and pepper chat fruit viroid.
Achieved acceptable analytical sensitivity and specificity for the detection of all targeted viruses and viroids.
Demonstrated robustness and reproducibility in virus detection across symptomatic plant samples.
Performance criteria met diagnostic requirements, facilitating reliable testing through in-house capabilities.

Porcher et al. (2026) studied this question.

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