What question did this study set out to answer?

This research aims to improve DNA barcoding techniques for timber species identification using herbarium collections.

June 14, 2026Applications in Plant SciencesOpen Access

Leveraging target enrichment and genome skimming (Hyb‐Seq) of herbarium collections to unlock timber DNA barcoding

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Authors

SBSidonie Bellot DBDyana Ndiade Bourobou CQCamila Quintero-Berns

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Overview

Randomized trial demonstrates effective DNA barcoding in timber species using enhanced sequencing methods, suggesting improved species identification.

Key Points

This research aims to improve DNA barcoding techniques for timber species identification using herbarium collections.
Developed a Hyb-Seq workflow for DNA barcoding in timber species.
Sequenced up to 350 nuclear and 177 plastid loci from 132 herbarium specimens.
Identified candidate barcodes after testing PCR amplification and Sanger sequencing on wood DNA.
Three candidate barcodes provided accurate species-level identifications with monophyly rates above 80%.
Identification issues with wood DNA were often linked to DNA impurity rather than degradation.
The workflow supports improved monitoring of plant diversity through existing herbarium data.

Cite This Study

Bellot et al. (2026) studied this question.

synapsesocial.com/papers/6a2e46b3b1cc60ccdea8b60d https://doi.org/10.1002/aps3.70063

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June 14, 2026Applications in Plant SciencesOpen Access

Leveraging target enrichment and genome skimming (Hyb‐Seq) of herbarium collections to unlock timber DNA barcoding

View Full Paper

Ask AI

Bookmark

Authors

SBSidonie Bellot DBDyana Ndiade Bourobou CQCamila Quintero-Berns

Discussion

Member takes

Overview

Randomized trial demonstrates effective DNA barcoding in timber species using enhanced sequencing methods, suggesting improved species identification.

Key Points

This research aims to improve DNA barcoding techniques for timber species identification using herbarium collections.
Developed a Hyb-Seq workflow for DNA barcoding in timber species.
Sequenced up to 350 nuclear and 177 plastid loci from 132 herbarium specimens.
Identified candidate barcodes after testing PCR amplification and Sanger sequencing on wood DNA.
Three candidate barcodes provided accurate species-level identifications with monophyly rates above 80%.
Identification issues with wood DNA were often linked to DNA impurity rather than degradation.
The workflow supports improved monitoring of plant diversity through existing herbarium data.

Cite This Study

Bellot et al. (2026) studied this question.

synapsesocial.com/papers/6a2e46b3b1cc60ccdea8b60d https://doi.org/10.1002/aps3.70063

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