Randomized trial demonstrates genetic modification in zebrafish, suggesting new model generation techniques.
Haploid embryonic stem cells (haESCs) from multiple species, including medaka fish, 1 mouse, 2 , 3 rat, 4 cattle, sheep, 5 and human, 6 can “fertilize” oocytes to produce semi-cloned embryos/animals and thus have potential applications in generating animal models with complex modifications. In mice, androgenetic haESCs (AG-haESCs) carrying both H19 -DMR and IG-DMR deletions can efficiently support the generation of semi-cloned pups upon intracytoplasmic AG-haESC injection (ICAHCI) into oocytes, at rates of up to 30.0% of transferred embryos. Therefore, mouse DKO-AG-haESCs have been used as a genetically tractable fertilization tool for complex genetic analyses at the organismal level. 7 This approach, however, has remained unavailable in zebrafish, a key model organism for studying vertebrate development and diseases.
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Ai et al. (2026) studied this question.
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