Key result
Perilla frutescens seed extract inhibits osteoclast differentiation and boosts bone strength in ovariectomized mice.
Why the study?
The defatted residue of Perilla frutescens seeds remains largely underutilized in food applications, prompting investigation into its effects on osteoclast differentiation and bone-related changes.
Hot-water extract of defatted Perilla frutescens seed residue suppresses osteoclast differentiation and improves bone parameters in ovariectomized mice, suggesting potential benefits for bone health.
May inhibit osteoclastogenesis in vitro; hypothesis-generating data leave open any role in bone disorders pending in vivo studies.
The defatted residue of Perilla frutescens seeds, a by-product of edible oil production, remains largely underutilized in food applications. This study investigated the effects of a hot-water extract derived from this residue on osteoclast differentiation and bone-related changes. In bone marrow-derived macrophages, the extract inhibited receptor activator of nuclear factor-κB ligand-induced osteoclast differentiation, as evidenced by the reduced formation of tartrate-resistant acid phosphatase-positive multinucleated cells and decreased tartrate-resistant acid phosphatase activity, without significantly affecting cell viability. In ovariectomized mice, the extract increased trabecular region length and cortical bone thickness. In a separate experiment, it enhanced femoral mechanical strength in a three-point bending test. High-performance liquid chromatography analysis identified rosmarinic acid and salviaflaside in the extract. These findings suggest that defatted Perilla frutescens seed residue may be a functional resource for improving bone health.
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Asano et al. (2026) studied Bone loss (ovariectomized mice model). Hot-water extract of defatted Perilla frutescens seed residue vs. Control was evaluated on Osteoclast differentiation, trabecular region length, cortical bone thickness, and femoral mechanical strength. Hot-water extract of defatted Perilla frutescens seed residue inhibited osteoclast differentiation in vitro and increased trabecular region length, cortical bone thickness, and mechanical strength in ovariectomized mice.
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