Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 15, 2026ACS SensorsOpen Access

Polyethylene Glycol Induced Condensation Leads to an Anomalous FRET Response from a Flexible Linker-Fluorescent Protein Crowding Sensor

View Full Paper
Ask AI
Bookmark
Share

Authors

AMAnshuman MohapatraJAJeanpun AntarasenDLDanielle R. Latham

Discussion

Loading...

Member takes

Overview

Randomized trial investigates how PEG-driven phase separation influences FRET responses in cytosolic crowding, suggesting new sensor development is necessary.

Key Points

  • This research aims to explore how polyethylene glycol (PEG) driven phase separation affects the response of fluorescent protein crowding sensors.
  • Investigated the phase separation of AcGFP1/mCherry-FRET crowding sensor and DNA-based crowding sensor under 100-400 mg/mL PEG conditions.
  • Used fluorescence recovery after photobleaching imaging and two-color fluorescence microscopy for analysis.
  • Compared the excluded volume effects of PEG and Ficoll on sensor responses at various concentrations.
  • ≥18% PEG (w/v) induced phase separation of CrH2, altering its sensor response.
  • CrD formed fluorescence puncta after charge-neutralization with poly-l-lysine, suggesting interactions influencing detection.
  • Fluorescent puncta exhibited liquid-like properties and inhomogeneity, revealing limitations of bulk fluorometry measurements.

Cite This Study

Mohapatra et al. (2026) studied this question.

synapsesocial.com/papers/6a2f96b4a1cfeec49082803chttps://doi.org/10.1021/acssensors.5c03872
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1BPS2026 – Chemical interactions in polyethylene glycol-induced phase separation lead to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor2026
  2. 2Polyethylene Glycol-Mediated Crowding: Insights through Protein Dynamics and Phase Separation2026 · 1 citations
  3. 3BPS2026 – FRET analysis of mCerulean3-linker-mCitrine constructs using two-photon time-resolved fluorescence: How crowding agent size and surface charge influence conformational dynamics2026
  4. 4PEG-mCherry interactions beyond classical macromolecular crowding2024 · 3 citations
  5. 5Effect of molecular crowding on native Cytochrome C: A Time-Dependent study2026