Investigation of the Photobiomodulation Effects on Viability and Wound Healing Activity in Human Umbilical Vein Endothelial Cells (HUVEC)

With recent advancements in stem cell-based therapeutic methods and novel techniques, such as photobiomodulation (PBM), a recognized safe approach with remarkable therapeutic potential, this study examined the cytotoxic effects of laser irradiation and its impact on wound healing in human umbilical vein endothelial cells (HUVECs). HUVEC were obtained from the Pasteur Institute and cultured under appropriate conditions. Cell cytotoxicity was assessed 24 and 48 h post-laser irradiation (660 and 980 nm) using the MTT assay. To evaluate cellular migration, a wound healing assay was performed, and images of the migration phase were captured. The results indicated that there was no statistically significant difference in the absorbance (570 nm) between the two time points (24 and 48 h) (p > 0.05). Additionally, imaging of cell migration at intervals of 0, 24, and 48 h demonstrated that while cell migration was observed in samples exposed to both 660 nm and 980 nm wavelengths, the most significant migration rate was noted at the 48-hour mark in samples treated with the 660 nm laser. These findings suggest that stimulation of cells with photobiomodulation can significantly enhance the wound healing activity of vascular endothelial cells, positioning it as a novel therapeutic method.