Antifungal activity of the Ocimum sanctum against Alternaria solani and Fusarium oxysproum

We evaluated the antifungal activity of Ocimum sanctum (Tulsi), Lamiaceae family) a medicinal plant with therapeutic properties, to inhibit the growth of plant pathogenic fungi (Alternaria solani and Fusarium oxysproum). Crude extracts of its stem, leaf and seed were prepared in distilled water using the maceration method. Using food poison method, plant parts extracts in various concentrations (6.25, 11.76 and 16.66 mg/ml) applied to assess their in-vitro antifungal activity. The fungicidal chemical components of plant extracts were analysed using UV-Visible spectroscopy, FTIR, Gas Chromatography and Mass Spectroscopy and study their active antifungal components (1-Isobutylsulfany lmethyl-2,8,9-trioxa-5-aza-1-sila-bicyclo[3.3.3] undecane, Methyl stearate, Methanimidmide, N,N-dimethyl-N'-phenyl-, 2,4-Di-tert-butylphenol and 7,9-Di-tert-butyl-1-oxaspiro (4,5)deca-6,9-diene-2,8-dione) inhibiting the growth of fungi. The aqueous extract (6.25 mg ml⁻¹) of Ocimum sanctum exhibited maximum inhibition of 30.0% against Alternaria solani and 31.3% against Fusarium oxysporum than control.