What is the clinical evidence from this study?

Study design: Other. Intervention: Bacterially produced TM isoforms (TM-2, TM-3, TM-5a, TM-5b). Primary outcome: Intracellular localization and F-actin binding.

August 15, 1992The Journal of Cell BiologyOpen Access

In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a, and TM-5b are co-localized in interphase fibroblasts.

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Key result

Bacterially produced tropomyosin isoforms TM-2, TM-3, TM-5a, and TM-5b bind to F-actin in vitro and co-localize along actin filaments in rat fibroblasts without preferred cellular locations.

Population

Rat fibroblasts and bacterially produced tropomyosin isoforms (TM-2, TM-3, TM-5a, TM-5b)

Design

Preclinical

Authors

MPMark F. PittengerUniversity of Maryland, Baltimore DHDavid M. HelfmanKorea Advanced Institute of Science and Technology

Discussion

Member takes

Implication

Bacterially produced tropomyosins validate as research tools; leaves open isoform roles in cardiac fibroblasts.

Structured PICO

Population

Rat fibroblasts and bacterially produced tropomyosin isoforms (TM-2, TM-3, TM-5a, TM-5b)

Intervention

Microinjection of fluorescently derivatized TM isoforms into rat fibroblasts

Outcome

Intracellular localization and F-actin binding properties

Bacterially produced tropomyosin isoforms from the alpha gene co-localize along actin filaments in rat fibroblasts without preference for specific subsets or locations.

Cite This Study

Pittenger et al. (1992) studied this question. Bacterially produced TM isoforms (TM-2, TM-3, TM-5a, TM-5b) was evaluated on Intracellular localization and F-actin binding. Bacterially produced tropomyosin isoforms TM-2, TM-3, TM-5a, and TM-5b bind to F-actin in vitro and co-localize along actin filaments in rat fibroblasts without preferred cellular locations.

synapsesocial.com/papers/6a309946912b1f0ec6bcaeef https://doi.org/10.1083/jcb.118.4.841

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In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a, and TM-5b are co-localized in interphase fibroblasts.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a, and TM-5b are co-localized in interphase fibroblasts.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider