Total Chemical Synthesis of a 304 Amino Acid K48‐Linked Tetraubiquitin Protein

A new record: The largest chemically synthesized polypeptide composed of 304 residues, which corresponds to folded K48-tetraubiquitin, has been achieved (see scheme). The presented synthetic method could be applied to any of the remaining tetraubiquitin chains, and should ultimately assist ongoing efforts to unravel how the remarkable diversity of ubiquitin signaling is achieved. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.