Key result
Aldosterone-producing adenoma tissues show markedly lower ANP binding capacity versus normal adrenal tissues.
Why the study?
Aldosterone secretion in aldosterone-producing adrenocortical adenomas failed to be suppressed by human atrial natriuretic peptide in previous in vivo and in vitro studies.
Do aldosterone-producing adenoma cells have reduced atrial natriuretic peptide binding sites compared to normal adrenal tissue?
Do aldosterone-producing adenoma cells have reduced atrial natriuretic peptide binding sites compared to normal adrenal tissue?
The refractoriness of aldosterone-producing adenomas to atrial natriuretic peptide suppression may be driven by a reduction in specific ANP receptor sites.
Supports further exploration of ANP signaling in adenomas; does not yet inform clinical practice.
The previous study demonstrated that the aldosterone secretion of aldosterone-producing adrenocortical adenoma failed to be suppressed by human atrial natriuretic peptide, based on the data in synthetic α-human atrial natriuretic peptide infusion in vivo and the effect on in vitro secretion from cultured adenoma cells. Using the membrane fractions prepared from human adrenal tissues and an aldosterone-producing adenoma tissues, we characterized the binding sites for [^<125>I] α-human atrial natriuretic peptide by the binding study and affinitylabeling of [^<125>I] α-human atrial natriuretic peptide. Both normal adrenal and adenoma tissue membrane fractions possessed specific binding sites, however the relative binding capacity for [^<125>I] α-human atrial natriuretic peptide in the adenoma preparations was markedly lower than that in the normal adrenal tissue preparation. The specific binding sites for [^<125>I] α-human atrial natriuretic peptide were detected at the region of 140 KD and 67-70 KD only in the normal adrenal membrane fractions. Our data suggest that the refractoriness to the effect of atrial peptide may be due to the reduced receptor sites in aldosterone-producing adenoma cells.
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Ōhashi et al. (1991) studied Aldosterone-producing adrenocortical adenoma. Aldosterone-producing adenoma tissue vs. Normal adrenal tissue was evaluated on Specific binding sites and relative binding capacity for [^125I] alpha-human atrial natriuretic peptide. Aldosterone-producing adenoma tissues exhibited markedly lower relative binding capacity for alpha-human atrial natriuretic peptide compared to normal adrenal tissues.
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