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January 24, 2024Virology JournalOpen Access

Porcine epidemic diarrhea virus strain CH/HLJ/18 isolated in China: characterization and phylogenetic analysis

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Key result

Recombinant PEDV strain CH/HLJ/18 causes 100% mortality and severe diarrhea in newborn piglets.

  • n=8

Why the study?

Porcine epidemic diarrhea virus distribution and pathogenicity have increased in China, requiring a better understanding of its genetic evolution and diversity for vaccine development, prevention, and treatment.

Population

Newborn piglets and Vero cell cultures

Comparison

Inoculation with PEDV strain CH/HLJ/18

Design

In vitro isolation, phylogenetic analysis, and in vivo animal inoculation study

Follow-up

7 d

Authors

YGYuyao GuoLSLing SuiDKDe‐Ming Kong

Discussion

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Overview

Supports PEDV genomic surveillance in China; leaves open functional and vaccine implications for GII-a strains.

Structured PICO

P
Population
8 newborn piglets were randomly allocated to evaluate the pathogenicity of the newly isolated PEDV CH/HLJ/18 strain over 7 days.
I
Intervention
Inoculation with PEDV strain CH/HLJ/18 (5 mL of 5.1 × 10³ PFU/mL)
C
Comparator
Sham-inoculated control group fed 5 mL of M199 medium
O
Outcome
Pathogenicity evaluated by clinical signs (diarrhea, vomiting), mortality, and histopathological lesions

Main Result

Absolute Event Rate: 100% vs 0%

The isolation and characterization of the highly pathogenic recombinant PEDV strain CH/HLJ/18 provides insights into the genetic evolution of PEDV in China.

Cite This Study

Guo et al. (2024) studied Porcine epidemic diarrhea (n=8). PEDV CH/HLJ/18 strain vs. Sham-inoculated control (5 mL M199) was evaluated on Mortality within 7 days. The newly isolated recombinant PEDV strain CH/HLJ/18 was highly pathogenic, causing severe watery diarrhea and 100% mortality within 7 days in inoculated newborn piglets.

synapsesocial.com/papers/6a318203eaf4e7f5219f7a02https://doi.org/10.1186/s12985-023-02233-6
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1PEDV ORF3 encodes an ion channel protein and regulates virus production2012 · 193 citations
  2. 2Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus2015 · 680 citations
  3. 3Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 20212022 · 75 citations
  4. 4PEDV: Insights and Advances into Types, Function, Structure, and Receptor Recognition2022 · 114 citations
  5. 5The 3.1-Angstrom Cryo-electron Microscopy Structure of the Porcine Epidemic Diarrhea Virus Spike Protein in the Prefusion Conformation2019 · 115 citations