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High Resolution Image Download MS PowerPoint Slide This study introduces a novel investigation of the interaction between Komagataella phaffii cells and iron oxide-based magnetic nanoparticles (Fe 3 O 4 MNPs) via protein secretion and machine learning (ML)-assisted surface-enhanced Raman scattering (SERS). For the first time, we produced Fe 3 O 4, Fe 3 O 4 @PEG, Fe 3 O 4 @PEI 10kDa, and Fe 3 O 4 @PEI 25kDa MNPs by a one-pot coprecipitation reaction. The addition of polymers to the reaction conditions significantly affected the shape, surface charge, size, and size distribution of the MNPs. The surface modification of MNPs is effectively accomplished using polyethylenimine (PEI), and the ζ-potential values of the MNPs exceed +25 mV under the NH 4 OH control. The homogeneity of MNPs synthesized with NH 4 OH is more pronounced according to transmission electron microscopy (TEM) pictures. All MNPs exhibited excellent immobilization efficiency (>92%) when we used 250 ppm Fe-containing MNP solutions. Smaller MNPs uniformly encapsulated the surface of K. phaffii cells, whereas larger MNPs exhibited irregular accumulation. K. phaffii cells exhibited excellent viability in all MNP solutions at up to 1000 ppm of Fe concentrations. Finally, the highest recombinant azurin protein secretion rate was obtained in Fe 3 O 4 @PEI 10kDa MNP-immobilized cells (about 1.3 times). The ML-assisted SERS analysis revealed that MNP interactions with K. phaffii cells were mediated by proteins such as mannoproteins and membrane transporter proteins as well as N-acetylglucosamine (i.e., chitin). These findings revealed the effect of the size and surface properties of MNPs on the immobilization of K. phaffii cells and the enormous potential of magnetic immobilization for protein secretion.
Dağcı et al. (Wed,) studied this question.