Transforming PRP into a shelf-stable therapy: Lyophilization preserves angiogenic and regenerative properties.

Objectives: Platelet-rich plasma (PRP) is widely used in regenerative medicine due to its high concentration of bioactive growth factors; however, its short shelf life limits its clinical applicability. Lyophilization has been proposed to extend PRP stability, but its impact on biological efficacy remains unclear. This study evaluated whether lyophilization alters the biochemical composition and regenerative function of PRP derived from expired platelet units unsuitable for transfusion. Materials and Methods: ), and CD31 immunocytochemistry at 5% and 10% concentrations, with fetal bovine serum (FBS) as a control. Results: 0.05). Conclusion: Despite a moderate reduction in growth factors, lyophilized PRP retained regenerative efficacy, supporting lyophilization as a viable strategy for the stable, sustainable reuse of PRP.