Biotinylated Silatrane: Development Functional Organosilicon Biointerfaces for Molecular Detection

H NMR, FT-IR, and mass spectrometry. To develop a multifunctional biointerface, biotinylated silatrane was co-deposited with a zwitterionic sulfobetaine silatrane, creating a dual functional coating that integrates specific molecular recognition with potent antifouling properties. Surface characterization via water contact angle (WCA) goniometry, spectroscopic ellipsometry, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM) confirmed the formation of highly hydrophilic, uniform, and stable organosilicon adlayers. The specificity of the platform was validated through avidin bioconjugation, where quartz crystal microbalance with dissipation (QCM-D) and interfacial analysis confirmed the formation of a stable, well-ordered avidin layer. Finally, the biotinylated silatrane-based architecture was successfully employed for the detection of cancer biomarker carcinoembryonic antigen (CEA), demonstrating a linear response at clinically relevant concentrations. The findings establish biotinylated silatrane as a robust and versatile building block for the development of high-performance and fouling-resistant biointerfaces in diagnostic technologies and biosensing platforms.