STAR Protocols question-and-answer series: RNA-seq

RNA sequencing (RNA-seq) is a powerful technique that allows researchers to study gene expression at a genome-wide level in various biological systems. Advances in RNA-seq protocols and data analysis have improved the accuracy and reliability of gene expression measurements. However, RNA-seq experiments and data analysis can be technically complex and demanding, and researchers may encounter various challenges. In this backstory, STAR Protocols authors and advisory board members share their advice on common questions about RNA-seq.