Quantitative Analysis of the Intracellular Efficacy of Conventional Antileishmanial Drugs Against Leishmania tropica: A Translational Approach Using the THP-1 Macrophage-Amastigote Model

Objective: conditions, and subsequent luminometric analysis. Methods: ) of transformed promastigotes, with microscopic enumeration of amastigotes in Giemsa-stained slides used for validation. Inhibitory concentration 50 (IC50) values were calculated from dose-response curves and compared using Student's t-test and Pearson's correlation coefficient. Results: Amphotericin B and pentamidine showed the highest efficacy; miltefosine demonstrated moderate activity; antimonials exhibited relatively low efficacy. IC50 values obtained by luminometric and microscopic methods were highly concordant: the paired t-test revealed no significant difference (t=0.2172, p=0.8387), and Pearson's correlation confirmed a strong positive relationship (r=0.9944, p=0.005). Conclusion: .