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June 17, 2026PhytonOpen Access

Optimization of Culture Medium and Conditions for In Vitro Pollen Germination in Hibiscus mutabilis Linn.

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Authors

SFSiyi FangBZBin ZhengJMJiao Ma

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Implication

Randomized trial evaluates optimal conditions for pollen germination in Hibiscus mutabilis, indicating improvements for breeding and conservation.

Key Points

  • This research aims to determine the best culture medium and environmental conditions for in vitro pollen germination and pollen tube growth in Hibiscus mutabilis.
  • Used Brewbaker and Kwack medium with varying concentrations of sucrose, H3BO3, CaCl2, MgSO4·7H2O, and KNO2.
  • Investigated environmental factors including temperature, light intensity, humidity, and pH.
  • Identified optimal concentrations: 300 g/L sucrose, 50 mg/L H3BO3, and 150 mg/L CaCl2.
  • Achieved maximum germination rates of 69.3% for red pollen and 65.7% for white pollen.
  • Optimal conditions included 20°C, 100% humidity, and slightly acidic pH of 6.1.
  • Dark conditions improved germination success, while moderate light intensity (6000-12000 lux) enhanced pollen tube elongation.

Cite This Study

Fang et al. (2026) studied this question.

synapsesocial.com/papers/6a323c29d50b63ecad206642https://doi.org/10.32604/phyton.2026.083644
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Also Consider

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  1. 1Effect of pollen morphology on hybridization and seed setting in hibiscus (Hibiscus rosa sinensis)2024
  2. 2Optimization and Development of Pollen Germination Medium for Cultivated and Wild Cajanus Pollen in vitro2025
  3. 3Evaluating the precision of staining methods in determining pollen viability and in vitro pollen germination in two endemic varieties of Humboldtia brunonis Wall. (Fabaceae-Detarioideae)2024
  4. 4Pollination biology and reproductive adaptation of Hibiscus mutabilis Linnaeus (Malvaceae): seasonal variation in floral traits, pollinator assemblages, and breeding system2026
  5. 5Optimized Protocol for in vitro Pollen Germination in Brinjal (Solanum melongena L.)2024