A Reagent-Free Binding Assay for Cholera Toxin Based on Sequestration Electrochemistry Using Lactose-Functionalized Magnetic Microbeads

Mariwo Ikeuchi; K. S. Shalini Devi; Kazuto Sazawa; Kazuharu Sugawara; Hideki Kuramitz

doi:10.1039/d6an00392c

January 1, 2026The Analyst

A Reagent-Free Binding Assay for Cholera Toxin Based on Sequestration Electrochemistry Using Lactose-Functionalized Magnetic Microbeads

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Key Points

The study aims to develop a reagent-free electrochemical binding assay for cholera toxin using functionalized magnetic microbeads.
Developed a one-step electrochemical assay without reagents.
Utilized magnetic microbeads functionalized with pyrroloquinoline quinone (PQQ) and lactose as the ligand.
Assayed for cholera toxin using the established method.
Demonstrated effective binding of cholera toxin to lactose-functionalized microbeads.
Achieved significant electrochemical signal change indicating toxin presence.

Implication

Evaluates electrochemical binding of cholera toxin in a reagent-free approach, indicating a novel diagnostic tool.

Abstract

We established a reagent-free one-step electrochemical binding assay for cholera toxin (CT) using magnetic microbeads functionalized with pyrroloquinoline quinone (PQQ) as an electroactive compound and lactose as the ligand for...

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Cite This Study

Ikeuchi et al. (2026) studied this question.

synapsesocial.com/papers/6a323c29d50b63ecad2066c6 https://doi.org/10.1039/d6an00392c

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A Reagent-Free Binding Assay for Cholera Toxin Based on Sequestration Electrochemistry Using Lactose-Functionalized Magnetic Microbeads

Key Points

Implication

Abstract

Discussion

Cite This Study

A Reagent-Free Binding Assay for Cholera Toxin Based on Sequestration Electrochemistry Using Lactose-Functionalized Magnetic Microbeads

Key Points

Implication

Abstract

Discussion

Cite This Study

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