Controlled immobilization of single-domain antibodies using cellulose-binding modules for AB ₅ -type toxin detection in paper-based assays

Q: What does this research mean for the field?

A single-domain antibody (VHH)-based lateral flow assay utilizing cellulose-binding modules enables the detection of AB5-type toxins, specifically cholera toxin and heat-labile enterotoxin, in human fecal and environmental water samples. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Selma B. Belfakir; Jonas A. Jürgensen; Marcus Petersson; Max Stæhr Wraae; Patrick Munk; Suthimon Thumtecho; Mateus G. Masteghin; Anne Ljungars; Andreas H. Laustsen; Sandra W. Thrane; Georgina M.S. Ross

doi:10.1039/d6ra01063f

January 1, 2026RSC AdvancesOpen Access

Controlled immobilization of single-domain antibodies using cellulose-binding modules for AB ₅ -type toxin detection in paper-based assays

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Key Points

This research aims to create an effective method for detecting specific toxins using single-domain antibodies and paper-based assays.
Developed a sandwich-format lateral flow assay using single-domain antibodies.
Tested the assay on human fecal samples and environmental water samples.
Utilized cellulose-binding modules for immobilizing the antibodies.
Successfully detected cholera toxin from V. cholerae in samples.
Detected heat-labile enterotoxin from enterotoxigenic E. coli across various sample types.

Implication

Develops a lateral flow assay for cholera and heat-labile enterotoxin detection, indicating broad applications.

Abstract

A single-domain antibody (VHH)-based sandwich-format lateral flow assay was developed for the detection of cholera toxin from V. cholerae and heat-labile enterotoxin from enterotoxigenic E. coli in human fecal and environmental water samples.

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Cite This Study

Belfakir et al. (2026) studied this question.

synapsesocial.com/papers/69edacdb4a46254e215b4836 https://doi.org/10.1039/d6ra01063f

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Controlled immobilization of single-domain antibodies using cellulose-binding modules for AB ₅ -type toxin detection in paper-based assays

Key Points

Implication

Abstract

Discussion

Cite This Study

Controlled immobilization of single-domain antibodies using cellulose-binding modules for AB ₅ -type toxin detection in paper-based assays

Key Points

Implication

Abstract

Discussion

Cite This Study

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