The production and properties of cholera Inaba-specific monoclonal antibodies

Fusion of hyperimmune mouse splenocytes and NSO myeloma cells gave rise to a kit of hybridomas producing monoclonal antibodies (MAb) specific to Vibrio cholerae O1 serovar Inaba. The study of the properties of MAb showed that the latter of hybridomas A5 and E9 belonging to IgG and IgM, respectively, met the diagnostic requirements. The MAb preparations precipitated from hybridoma culture fluids may be used to identify only Vibrio cholerae O1 serovar Inaba in the slide agglutination and indirect immunofluorescence tests and dot immunoassay. The immunoblotting data suggest that Inaba-specific MAbs are directed towards the epitopes located in the polysaccharide chain of the polysaccharide from Vibrio Inaba. The resultant MAbs may be themselves used in serological tests for cholera and employed to design novel more perfect, diagnostic agents.