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The study aimed to create accurate diagnostic tools for monkeypox virus using novel monoclonal antibodies targeting the A29L protein.

June 17, 2026Microbiology SpectrumOpen Access

Development and preliminary clinical validation of A29L mAb-based ELISA and lateral flow immunoassays for monkeypox virus detection

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The study aimed to create accurate diagnostic tools for monkeypox virus using novel monoclonal antibodies targeting the A29L protein.
Developed a panel of six high-affinity monoclonal antibodies against A29L through immunization and hybridoma screening.
Created a sensitive ELISA with a detection limit of 31.25 pg/mL and conducted clinical evaluations on patient samples.
Developed a rapid lateral flow immunoassay for point-of-care testing with clinical evaluations using plasma and vesicular fluid samples.
The ELISA showed 95% sensitivity and 100% specificity for plasma samples, closely matching gold-standard PCR results.
The lateral flow immunoassay demonstrated sensitivities of 58.3% and 40.0% for plasma and vesicular fluid respectively, with perfect detection in high viral load cases.

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Randomized trial develops rapid LFIA and sensitive ELISA for monkeypox virus detection, enhancing outbreak response efforts.

Liu et al. (2026) studied this question.

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