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This study evaluates the variability in salt tolerance among eight alfalfa varieties under different NaCl levels.

June 17, 2026BMC Plant BiologyOpen Access

Assessment of Alfalfa varieties under salt stress through morpho-physiological and biochemical traits using a comprehensive membership function approach

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Randomized trial assesses salt tolerance in alfalfa varieties, indicating implications for breeding salt-resistant crops.

This study evaluates the variability in salt tolerance among eight alfalfa varieties under different NaCl levels.
Used four NaCl levels (0, 60, 120, 180 mM) to assess responses.
Employed membership function analysis, correlation analysis, and PCA to evaluate traits.
Measured morphological, physiological, and biochemical traits of each variety.
Plant height, shoot dry weight, root dry weight, and number of leaves decreased by up to 61.36% under high salt stress.
Electrolyte leakage and malondialdehyde increased significantly (267.35% and 178.48%, respectively).
Variety V5 (A'erjienong) showed the highest salt tolerance, while V4 (Fudina) exhibited the highest sensitivity.

Daud et al. (2026) studied this question.

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