What question did this study set out to answer?

This research aims to investigate the effects of etomoxir on T cell proliferation and oxidative metabolism, particularly regarding its inhibition of CPT1a.

April 13, 2018Scientific ReportsOpen Access

The CPT1a inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations

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Authors

RORoddy S. O’ConnorUniversity of Pennsylvania LGLili GuoFujian Medical University SGSaba GhassemiUniversity of Pennsylvania

Discussion

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Implication

Randomized trial demonstrates that etomoxir induces oxidative stress in T cells, suggesting caution in its use for cellular studies.

Key Points

This research aims to investigate the effects of etomoxir on T cell proliferation and oxidative metabolism, particularly regarding its inhibition of CPT1a.
Evaluated T cell proliferation and memory differentiation following costimulation through CD28 receptor and etomoxir application.
Used shRNA to reduce CPT1a in T cells and assess effects on oxidative metabolism.
Investigated oxidative stress markers and reactive oxygen species production at varying concentrations of etomoxir.
Etomoxir (above 5 μM) significantly increases reactive oxygen species (ROS) generation, indicating severe oxidative stress in T cells.
T cell proliferation is moderately affected by etomoxir, with no significant impact on memory differentiation.
Oxidative metabolism in T cells relies more on glutamine than on fatty acid oxidation, illustrating etomoxir's non-specific effects.

Cite This Study

O’Connor et al. (2018) studied this question.

synapsesocial.com/papers/6a32ac03af034259ce4fa355 https://doi.org/10.1038/s41598-018-24676-6

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April 13, 2018Scientific ReportsOpen Access

The CPT1a inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations

View Full Paper

Ask AI

Bookmark

Authors

RORoddy S. O’ConnorUniversity of Pennsylvania LGLili GuoFujian Medical University SGSaba GhassemiUniversity of Pennsylvania

Discussion

Member takes

Implication

Randomized trial demonstrates that etomoxir induces oxidative stress in T cells, suggesting caution in its use for cellular studies.

Key Points

This research aims to investigate the effects of etomoxir on T cell proliferation and oxidative metabolism, particularly regarding its inhibition of CPT1a.
Evaluated T cell proliferation and memory differentiation following costimulation through CD28 receptor and etomoxir application.
Used shRNA to reduce CPT1a in T cells and assess effects on oxidative metabolism.
Investigated oxidative stress markers and reactive oxygen species production at varying concentrations of etomoxir.
Etomoxir (above 5 μM) significantly increases reactive oxygen species (ROS) generation, indicating severe oxidative stress in T cells.
T cell proliferation is moderately affected by etomoxir, with no significant impact on memory differentiation.
Oxidative metabolism in T cells relies more on glutamine than on fatty acid oxidation, illustrating etomoxir's non-specific effects.

Cite This Study

O’Connor et al. (2018) studied this question.

synapsesocial.com/papers/6a32ac03af034259ce4fa355 https://doi.org/10.1038/s41598-018-24676-6

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