Hyaluronic acid coating significantly reduced platelet deposition on stainless steel stents (0.82 vs 1.83 x 10^9 platelets; P<0.02) and tubes compared with uncoated controls.
Does hyaluronic acid coating reduce platelet thrombus formation on endovascular devices in a baboon thrombosis model?
Hyaluronic acid coating significantly reduces platelet thrombus formation on stainless steel stents and tubes in a primate model, suggesting a potential strategy for improving thromboresistance of endovascular devices.
Absolute Event Rate: 0.82% vs 1.83%
p-value: p=<0.02
Biocompatible stent coatings may alleviate problems of increased (sub)acute thrombosis after stent implantation. Hyaluronic acid (HA), a ubiquitous, nonsulfated glycosaminoglycan, inhibits platelet adhesion and aggregation and prolongs bleeding when administered systemically. However, the effects of immobilized HA for reducing stent platelet deposition in vivo are unknown. We therefore quantified the antithrombotic effects of coating stainless steel stents and tubes with HA using an established baboon thrombosis model under physiologically relevant blood flow conditions. HA-coated and uncoated (control) stents (3.5 mm in diameter, n=32) and stainless steel tubes (4.0 mm in diameter, n=18) were deployed into exteriorized arteriovenous shunts of conscious, nonanticoagulated baboons. Accumulation of (111)In-radiolabeled platelets was quantified by continuous gamma-camera imaging during a 2-hour blood exposure period. HA coating resulted in a significant reduction in platelet deposition in long (4 cm) tubes (0.24+/-0.15 x 10(9) versus 6.12+/-0.49 x 10(9) platelets; P<0.03), short (2 cm) stainless steel tubes (0.18+/-0.06 x 10(9) versus 3.03+/-0.56 x 10(9) platelets; P<0.008), and stents (0.82+/-0.20 x 10(9) versus 1.83+/-0. 23 x 10(9) platelets; P<0.02) compared with uncoated control devices. Thus, HA coating reduces platelet thrombus formation on stainless steel stents and tubes in primate thrombosis models. These results indicate that immobilized HA may represent an attractive strategy for improving the thromboresistance of endovascular devices.
Verheye et al. (Sat,) conducted a other in Thrombosis. Hyaluronic acid (HA) coating vs. Uncoated control devices was evaluated on Platelet deposition (accumulation of 111In-radiolabeled platelets on stents) (p=<0.02). Hyaluronic acid coating significantly reduced platelet deposition on stainless steel stents (0.82 vs 1.83 x 10^9 platelets; P<0.02) and tubes compared with uncoated controls.