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June 18, 2026Infection and Drug ResistanceOpen Access

Disseminated Cryptococcosis in a Non-HIV Patient: Diagnostic Value of Integrating mNGS, Culture, and Antigen Testing

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Authors

FHFengzhen HeYYYongcheng YangDMDuo Ma

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Overview

Case report demonstrates diagnostic value of mNGS, culture, and antigen testing for cryptococcosis in non-HIV patients, indicating challenges in diagnosis.

Key Points

  • This case report aims to illustrate the diagnosis of disseminated cryptococcosis in a non-HIV patient using various diagnostic methods.
  • Case report of a 72-year-old male post-cardiac surgery with disseminated cryptococcosis.
  • Integrated diagnostic approach using metagenomic next-generation sequencing, culture, and antigen testing.
  • Patient received liposomal amphotericin B and flucytosine antifungal treatment.
  • Cryptococcus neoformans was identified using mNGS with 8 sequence reads and cultured from blood, urine, and pleural effusion.
  • Cerebrospinal fluid tested positive for cryptococcal capsular polysaccharide antigen, confirming disseminated infection.
  • The patient improved initially but suffered from secondary multiorgan failure, leading to prolonged hospitalization of 98 days.

Cite This Study

He et al. (2026) studied this question.

synapsesocial.com/papers/6a338ba9630953a74978d3aahttps://doi.org/10.2147/idr.s597466
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